phospho erk1 2 ab Search Results


anti-phospho-erk1/2 (thr 202 /tyr 204 ) ab  (US Biological Life Sciences)
90
US Biological Life Sciences anti-phospho-erk1/2 (thr 202 /tyr 204 ) ab
Effect of transient knockdown of cPAcP by siRNA in LNCaP C-33 cells on tyrosine phosphorylation of ErbB-2. A, lysate proteins of transiently transfected cells with different amounts of PAcP siRNA-126 plasmids for 48 h were analyzed for cPAcP, cyclin D1, PCNA, and the phosphorylation (p) levels of ErbB-2, <t>p52Shc,</t> <t>ERK1/2,</t> Akt, Src, STAT3, and STAT5 proteins. After stripping the membrane, the protein levels of ErbB-2, Shc, ERK1/2, Akt, Src, STAT3, STAT5, and α-tubulin were detected, respectively. Similar results were obtained from five sets of independent experiments. B, LNCaP C-33 cells were plated at a cell density of 3 × 105/well in 6-well plates for 48 h and then transfected with different amounts of PAcP siRNA-126 plasmids. Control cells were transfected with the vector containing scramble oligonucleotides. Cell numbers were counted 3 days after transfection. The ratio of cell growth was calculated by normalizing the cell number to that of the control. The result shown is the average from three sets of independent experiments in duplicates (n = 2 × 3). Bars = S.E.
Anti Phospho Erk1/2 (Thr 202 /Tyr 204 ) Ab, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of transient knockdown of cPAcP by siRNA in LNCaP C-33 cells on tyrosine phosphorylation of ErbB-2. A, lysate proteins of transiently transfected cells with different amounts of PAcP siRNA-126 plasmids for 48 h were analyzed for cPAcP, cyclin D1, PCNA, and the phosphorylation (p) levels of ErbB-2, p52Shc, ERK1/2, Akt, Src, STAT3, and STAT5 proteins. After stripping the membrane, the protein levels of ErbB-2, Shc, ERK1/2, Akt, Src, STAT3, STAT5, and α-tubulin were detected, respectively. Similar results were obtained from five sets of independent experiments. B, LNCaP C-33 cells were plated at a cell density of 3 × 105/well in 6-well plates for 48 h and then transfected with different amounts of PAcP siRNA-126 plasmids. Control cells were transfected with the vector containing scramble oligonucleotides. Cell numbers were counted 3 days after transfection. The ratio of cell growth was calculated by normalizing the cell number to that of the control. The result shown is the average from three sets of independent experiments in duplicates (n = 2 × 3). Bars = S.E.

Journal: The Journal of Biological Chemistry

Article Title: Human Prostatic Acid Phosphatase, an Authentic Tyrosine Phosphatase, Dephosphorylates ErbB-2 and Regulates Prostate Cancer Cell Growth *

doi: 10.1074/jbc.M109.098301

Figure Lengend Snippet: Effect of transient knockdown of cPAcP by siRNA in LNCaP C-33 cells on tyrosine phosphorylation of ErbB-2. A, lysate proteins of transiently transfected cells with different amounts of PAcP siRNA-126 plasmids for 48 h were analyzed for cPAcP, cyclin D1, PCNA, and the phosphorylation (p) levels of ErbB-2, p52Shc, ERK1/2, Akt, Src, STAT3, and STAT5 proteins. After stripping the membrane, the protein levels of ErbB-2, Shc, ERK1/2, Akt, Src, STAT3, STAT5, and α-tubulin were detected, respectively. Similar results were obtained from five sets of independent experiments. B, LNCaP C-33 cells were plated at a cell density of 3 × 105/well in 6-well plates for 48 h and then transfected with different amounts of PAcP siRNA-126 plasmids. Control cells were transfected with the vector containing scramble oligonucleotides. Cell numbers were counted 3 days after transfection. The ratio of cell growth was calculated by normalizing the cell number to that of the control. The result shown is the average from three sets of independent experiments in duplicates (n = 2 × 3). Bars = S.E.

Article Snippet: Anti-phospho-Erk1/2 (Thr 202 /Tyr 204 ) Ab was from United States Biological (Swampscott, MA).

Techniques: Transfection, Stripping Membranes, Plasmid Preparation